ad gfp u6 scrmb shrna  (Vector Biolabs)

Journal: JCI Insight

Article Title: CCN2 deficiency in smooth muscle cells triggers cell reprogramming and aggravates aneurysm development

doi: 10.1172/jci.insight.162987

Figure Lengend Snippet: Western blot analysis of various protein expression in cultured human primary aortic SMCs (HASMCs) with CCN2 deficiency (Ad-CCN2-shRNA versus Ad-scrmb-shRNA) ( A ) or overexpression (Ad-GFP-CCN2 versus Ad-GFP) ( B ). Target protein levels were normalized to GAPDH. Data were quantified and represented as mean ± SEM. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, 2-tailed Student’s t test.

Article Snippet: Adenoviruses used for CCN2 overexpression (Ad-GFP-hCTGF, ADV-206232) and knockdown (Ad-GFP-U6-h-CTGF-shRNA, shADV-206232) studies, henceforth AdCCN2 and shCCN2, and control viruses (Ad-GFP, Ad-GFP-U6-scrmb-shRNA), henceforth AdCtrl and shCtrl, were purchased from Vector Biolabs. siRNAs used for human TGF-β receptor knockdown (TGFBR1-7046, TGFFBR2-7048, and Nontargeting Pool) were purchased from Horizon. rhCCN2 (9190-cc) and TGF-β1 (rhTGF-β1, 240-B/CF) were purchased form R&D Systems and reconstituted in sterile PBS and 4 mM HCl-0.1% BSA, respectively, as recommended by the manufacturer.

Techniques: Western Blot, Expressing, Cell Culture, shRNA, Over Expression